| Reagent | Final Concentration | Volume (μL) |
|---|---|---|
| SuperScript™ III Reverse Transcriptase (200 U/μL) (Cat#18080044, Thermo Fisher Scientific) |
7.14 ng/μL | 27.5 |
| RNAsin Plus RNase Inhibitor (40 U/μL) (Cat#N2615, Promega) |
0.57 U/μL | 11 |
| 0.1 M DTT (supplied with SuperScript™ III Reverse Transcriptase) | 14.29 mM | 110 |
| dNTP (25 mM) (see Note blow) | 1.79 mM | 55 |
| 5× First-Strand Buffer (supplied with SuperScript™ III Reverse Transcriptase) | n/a | 330 |
| Nuclease-Free Water (Cat#129114, QIAGEN) | n/a | 236.5 |
| Total | n/a | 770 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.