Reagent	Final Concentration	Volume (μL)
HotStarTaq DNA Polymerase (5 U/μL) (Cat#203209, QIAGEN)	0.05 U/μL	42
Forward Primer Mixture (50 μM) (see Note above)	0.29 μM	23
Reverse Primer (50 μM) (see Note above)	0.19 μM	15
dNTP (25 mM) (see Note blow)	0.31 mM	48
10× PCR Buffer (supplied with HotStarTaq DNA Polymerase)	n/a	420
Nuclease-Free Water (Cat#129114, QIAGEN)	n/a	3,352
Total	n/a	3,900