PCR Cycling Conditions | |||
---|---|---|---|
Steps | Temperature | Time | Cycles |
Initial denaturation | 95°C | 15 min | 1 |
Denaturation | 94°C | 30 s | 50 cycles |
Annealing | 46°C | 30 s | |
Extension | 72°C | 55 s | |
Final extension | 72°C | 10 min | 1 |
Hold | 4°C | Forever |
PCR Cycling Conditions | |||
---|---|---|---|
Steps | Temperature | Time | Cycles |
Initial denaturation | 95°C | 15 min | 1 |
Denaturation | 94°C | 30 s | 50 cycles |
Annealing | 46°C | 30 s | |
Extension | 72°C | 55 s | |
Final extension | 72°C | 10 min | 1 |
Hold | 4°C | Forever |