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. 2020 Oct 16;1(3):100129. doi: 10.1016/j.xpro.2020.100129
PCR Cycling Conditions
Steps Temperature Time Cycles
Initial denaturation 95°C 15 min 1
Denaturation 94°C 30 s 50 cycles
Annealing 46°C 30 s
Extension 72°C 55 s
Final extension 72°C 10 min 1
Hold 4°C Forever