REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PE-Cy7 anti-human CD20 (Clone L27)	BD Biosciences	Cat#335811; RRID: AB_399985
Bv421 anti-human CD19 (Clone HIB19)	BD Biosciences	Cat#562440; RRID: AB_11153299
Bv421 anti-human IgG (Clone G18-145)	BD Biosciences	Cat#562581; RRID: AB_2737665
PE anti-human CD27 (Clone M-T271)	BD Biosciences	Cat#555441; RRID: AB_395834
APC anti-human IgG (Clone G18-145)	BD Pharmingen	Cat#550931; RRID: AB_398478
Alexa Fluor 488 anti-human CD19 (Clone HIB19)	BD Biosciences	Cat#557697; RRID: AB_396806
Biological Samples
Human Blood or Human Peripheral Blood Mononuclear Cells (PBMCs)	(Wang et al., 2020)	N/A
Chemicals, Peptides, and Recombinant Proteins
HBsAg adr CHO	ProSpec	Cat#HBS-875
Ovalbumin (257–264) Chicken	Sigma-Aldrich	Cat#S7951
Streptavidin-allophycocyanin (strep-APC)	BD Biosciences	Cat#554067; RRID: AB_10050396
Streptavidin-phycoerythrin (strep-PE)	eBioscience	Cat#12-4317-87
Streptavidin-Alexa Fluor 488	Thermo Fisher Scientific	Cat#S32354
PBS, pH 7.4	Thermo Fisher Scientific	Cat#10010023
PBS (10×), pH 7.2	Thermo Fisher Scientific	Cat#70013032
HyClone™ Fetal Bovine Serum	Fisher Scientific	Cat#SH3007103
Bovine Serum Albumin	Sigma-Aldrich	Cat#A0281
UltraPure™ 0.5 M EDTA, pH 8.0	Thermo Fisher Scientific	Cat#15575020
RPMI 1640 Medium	Thermo Fisher Scientific	Cat#11875093
Human Fc Block	BD Biosciences	Cat#564220
RNAsin Plus RNase Inhibitor	Promega	Cat#N2615
Nuclease-Free Water	QIAGEN	Cat#129114
DTT 0.1 M Solution	Thermo Fisher Scientific	Cat# 707265ML
IGEPAL® CA-630	Sigma-Aldrich	Cat#I8896
dATP Solution (100 mM)	Thermo Fisher Scientific	Cat#R0141
dTTP Solution (100 mM)	Thermo Fisher Scientific	Cat#R1191
dCTP Solution (100 mM)	Thermo Fisher Scientific	Cat#R0151
dGTP Solution (100 mM)	Thermo Fisher Scientific	Cat#R0161
UltraPure™ Sucrose	Thermo Fisher Scientific	Cat#15503022
Cresol Red	Sigma-Aldrich	Cat#114472
Critical Commercial Assays
EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit	Thermo Fisher Scientific	Cat#21935
EZ-Link™ Micro NHS-PEG4-Biotinylation Kit	Thermo Fisher Scientific	Cat#21955
CD19 MicroBeads, Human	Miltenyi Biotech	Cat#130-097-055
QuadroMACS™ Separator and Starting Kits	Miltenyi Biotech	Cat#130-091-051
LS Magnetic Columns	Miltenyi Biotech	Cat#130-042-401
Superscript III Reverse Transcriptase	Thermo Fisher Scientific	Cat#18080044
HotStarTaq DNA Polymerase	QIAGEN	Cat#203209
Oligonucleotides
F1-HC (5′-ACAGGTGCCCACTCCCAGG TGCAG)	(Wang et al., 2020)	N/A
F2-HC (5′-AAGGTGTCCAGTGTGARGT GCAG)	(Wang et al., 2020)	N/A
F3-HC (5′-CCCAGATGGGTCCTGTCCCAG GTGCAG)	(Wang et al., 2020)	N/A
F4-HC (5′-CAAGGAGTCTGTTCCGAGGT GCAG)	(Wang et al., 2020)	N/A
R1-HC (5′-GGAAGGTGTGCACGCCGCT GGTC)	(Wang et al., 2020)	N/A
R2-HC (5′-GTTCGGGGAAGTAGTCCTT GAC)	(Wang et al., 2020)	N/A
F1-Kappa (5′-ATGAGGSTCCCYGCTCA GCTGCTGG)	(Wang et al., 2020)	N/A
F2-Kappa (5′-CTCTTCCTCCTGCTACTC TGGCTCCCAG)	(Wang et al., 2020)	N/A
F3-Kappa (5′-ATTTCTCTGTTGCTCTGG ATCTCTG)	(Wang et al., 2020)	N/A
F4-Kappa (5′-ATGACCCAGWCTCCAB YCWCCCTG)	(Wang et al., 2020)	N/A
R1-Kappa (5′-GTTTCTCGTAGTCTGC TTTGCTCA)	(Wang et al., 2020)	N/A
R2-Kappa (5′-GTGCTGTCCTTGCT GTCCTGCT)	(Wang et al., 2020)	N/A
F1-Lambda (5′-GGTCCTGGGCCCAGTCT GTGCTG)	(Wang et al., 2020)	N/A
F2-Lambda (5′-GGTCCTGGGCCCAGTCT GCCCTG)	(Wang et al., 2020)	N/A
F3-Lambda (5′-GCTCTGTGACCTCCTAT GAGCTG)	(Wang et al., 2020)	N/A
F4-Lambda (5′-GGTCTCTCTCSCAGCYTG TGCTG)	(Wang et al., 2020)	N/A
F5-Lambda (5′-GTTCTTGGGCCAATTTTA TGCTG)	(Wang et al., 2020)	N/A
F6-Lambda (5′-GGTCCAATTCYCAGGCT GTGGTG)	(Wang et al., 2020)	N/A
F7-Lambda (5′-GAGTGGATTCTCAGACT GTGGTG)	(Wang et al., 2020)	N/A
R1-Lambda (5′-CACCAGTGTGGCCTTGT TGGCTTG)	(Wang et al., 2020)	N/A
R2-Lambda (5′-CTCCTCACTCGAGG GYGGGAACAGAGTG)	(Wang et al., 2020)	N/A
Random Primers	Thermo Fisher Scientific	Cat#48190011
Recombinant DNA
IGγ1 Expression Vector	(von Boehmer et al., 2016)	N/A
IGκ Expression Vector	(von Boehmer et al., 2016)	N/A
IGλ Expression Vector	(von Boehmer et al., 2016)	N/A
Software and Algorithms
IgBlast	(Ye et al., 2013)	http://www.ncbi.nlm.nih.gov/igblast/
IMGT/V-QUEST	(Brochet et al., 2008)	http://www.imgt.org/IMGT_vquest/vquest
Other
General Long-Term Storage Cryogenic Tubes	Nalgene	Cat#5000-1020
SimpliAmp™ Thermal Cycler	Thermo Fisher Scientific	Cat#A24811
500 mL Bottle Top Vacuum Filter, 0.22 μm Pore	CORNING	Cat#431118
Pipet-Lite Multi Pipette L12-20XLS+	RAININ	Cat#17013808