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Representative Example of the Steps for Quantifying Fluorescence of Intracellular Organelles Relative to Total Cell Areas
Acquire images in both DIC and in fluorescent channels of interest. DAPI (blue) can also be useful as a reference for counting total cell numbers per frame in alternative types of quantification. Right panels contain a pharmaceutical inhibitor and act as a negative control for the tracer of choice. Scale bar, 10 μm.
