REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
LysoLive (β-glucosidase substrate)	Marker Gene Tech	Cat# M27745
SiR-Lysosome	Cytoskeleton Inc	Cat# CYSC012
Lysosensor Yellow/Blue DND-160 (PDMPO)	ThermoFisher	Cat# L7545
Tetramethylrhodamine Dextran (TMR-Dx) 70,000 kDa	ThermoFisher	Cat# D1818
Bovine Serum Albumin DeQuenched (BSA-DQ)	ThermoFisher	Cat# D12051
Ovalbumin DeQuenched (DQ)	ThermoFisher	Cat# D12053
Lysotracker Deep Red	ThermoFisher	Cat# L12492
Alexa Fluor 488 Phalloidin	ThermoFisher	Cat# A12379
Cycloheximide	Sigma	Cat# C-7698
Lithium chloride (LiCl)	Sigma	Cat# L4408
CHIR99021	Sigma	Cat# SML1046
DMSO	Sigma	Cat# W387520
5-(N-Ethyl-N-isopropyl) amiloride (EIPA)	Sigma	Cat# A3085
IPA-3	Sigma	Cat# I2285
Sodium chloride (NaCl)	Sigma	Cat# S9888
Wnt3a	Peprotech	Cat# 315-20
Lipofectamine 3000	ThermoFisher	Cat# L3000001
Prolong Antifade	ThermoFisher	Cat#P36930
Prolong Antifade + DAPI	ThermoFisher	Cat# P36931
DMEM	ThermoFisher	Cat#11965092
RPMI 1640	ThermoFisher	Cat#12633012
Glutamine	ThermoFisher	Cat#25030081
Fetal Bovine Serum (FBS)	ThermoFisher	Cat#16000044
Bovine Serum Albumin (BSA)	ThermoFisher	Cat#9048468
Goat Serum	ThermoFisher	Cat#50197Z
Pen-Strep antibiotics	ThermoFisher	Cat#15140122
Triton X-100	ThermoFisher	Cat#HFH10
Fibronectin	ThermoFisher	Cat#33016015
Collagen	Sigma	Cat#C7661
10-cm dish	ThermoFisher	Cat#174903
12-well dish	ThermoFisher	Cat#150628
Circular coverslips	ThermoFisher	Cat# 12545100
8-well glass-bottom chamber slides	ibidi	Cat #80827
Experimental Models: Cell Lines
Alexander hepatocellular carcinoma	ATCC	RRID:CVCL_0485
Cos7	ATCC	RRID:CVCL_0224
HaCaT	ATCC	RRID:CVCL_3300
HeLa	ATCC	RRID:CVCL_0030
HEK293	ATCC	RRID:CVCL_0045
NIH-3T3	ATCC	RRID:CVCL_0594
SW480	ATCC	RRID:CVCL_0546
Recombinant DNA
LifeAct	IMSR	RRID:IMSR_EM:12427
pCS2-mGFP	Addgene	RRID:Addgene_14757
Software and Algorithms
Fiji imaging software	NIH	http://fiji.sc/
MaxEntropy PlugIn	NIH	http://imagej.nih.gov/ij/
Prism 7	GraphPad	http://www.graphpad.com/scientific-software/prism/
Imaris	Oxford	http://imaris.oxinst.com
Zen 2.3 imaging software	Zeiss	http://zeiss.com
Other
Axio Observer Z1 Inverted Microscope with Apotome	Zeiss	n/a