Figure 11.

Example of EBs that Failed to Inhibit the Extra-embryonic Hematopoietic Program

Faulty mesoderm patterning toward the definitive program in EBs at day 4 (left image) and day 7 (right image) of differentiation. Defective inhibition of the extra-embryonic hematopoietic program (failed SB/CHIR treatment) results in formation of stroma (adherent cells) and blood progenitor cells (round bright cells) around the EBs during the non-adherent culture (during the first 8 days of differentiation). Scale bars represent 100 μm.