Figure 7.

Formation of SOX17⁺ Vascular Endothelium

(A) Confocal images of combined Z-stack layers of EB cultures at day 10 and day 14 of differentiation of the SOX17^mCHERRY/wRUNX1C^GFP/w hESC line (Ng et al., 2016). At day 10, EBs have developed SOX17⁺ endothelium and are surrounded by outgrowths of stromal and SOX17⁺ endothelial cells, which will cover the entire well surface by day 14. Fluorescence and brightfield channels are merged. Scale bar represents 200 μm. SOX17 (mCHERRY, red).

(B) Epifluorescence microscopy images of EB cultures at day 14, displaying a monolayer of SOX17⁺ endothelium (upper panels) and SOX17⁺ vessels (bottom panels) growing from the EB structures. The first formed hematopoietic progenitors (SOX17^-) can be seen in cultures by day 14 (arrow). Panels show brightfield (left), epifluorescence (middle) and merged (right) fields. Scale bar represents 100 μm. SOX17 (mCHERRY, red).