Growth Factor | Final Concentration | Volume |
---|---|---|
STAPEL media | n/a | 100 mL |
BMP4 100 ng/μL | 10 ng/mL | 10 μL |
SCF 100 ng/μL | 100 ng/mL | 100 μL |
FLT3 100 ng/μL | 100 ng/mL | 100 μL |
TPO 100 ng/μL | 50 ng/mL | 50 μL |
VEGF 100 ng/μL | 50 ng/mL | 50 μL |
IL-6 100 ng/μL | 25 ng/mL | 25 μL |
IL-3 100 ng/μL | 25 ng/mL | 25 μL |
IGF2 100 ng/μL | 10 ng/mL | 10 μL |
FGF2 100 ng/μL | 10 ng/mL | 10 μL |
Pen/Strep (optional) | 1:200 | 500 μL |
Total | n/a | 100 mL |