| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC anti-human CD38 | BD Pharmingen | Cat # 555462; RRID: AB_398599 |
| APC anti-human CD90 | BD Pharmingen | Cat # 559869; RRID: AB_398677 |
| BV-421 anti-human CD34 | BioLegend | Cat # 343609; RRID: AB_11147951 |
| BV-421 anti-human CD43 | BD Horizon | Cat # 562916; RRID: AB_2737890 |
| BV-421 anti-human CD45 | BioLegend | Cat # 304032; RRID: AB_2561357 |
| BV-421 anti-human CD90 | BioLegend | Cat # 328121; RRID: AB_10933261 |
| PeCy7 anti-human CD16 | BioLegend | Cat # 302015; RRID: AB_314215 |
| PeCy7 anti-human CD34 | BioLegend | Cat # 343516; RRID: AB_1877251 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| α-MTG (α-Monothioglycerol) | Merck | Cat # M6145 |
| 2-Mercaptoethanol | Thermo Fisher Scientific | Cat # 21985-023 |
| AA2P (Ascorbic acid 2-phosphate) | Merck | Cat # A8960 |
| Accutase® solution | Merk | Cat # A6964 |
| Activin A | R&D Systems | Cat # 338-AC |
| BMP4 (Bone morphogenetic protein 4) | R&D Systems | Cat # 314-BP |
| Bovostar acid-stripped Bovine Serum Albumin | Bovogen, Australia (12 Williams Ave, Vic 3033) | Cat # BSAS 0.1 |
| CHIR99021 | Tocris Biosciences | Cat # 4423 |
| Collagenase Type 4 | Worthington | Cat # CLS-4 |
| Distilled water | Life Technologies | Cat # 15230-162 |
| DMEM/F12 (DMEM Nutrient Mixture F-12 1× + L-glutamine Na bicarbonate) | Thermo Fisher Scientific | Cat # 11320-033 |
| EDTA (Ethylenediaminetetraacetic acid), 0.5 M, UltraPure, pH 8.0 | Thermo Fisher Scientific | Cat # 15575020 |
| EtOH (Ethanol), 100%, AnalaR/Molecular biology grade | Merck | Cat # E7023 |
| FBS (Fetal Bovine Serum), Gibco™, qualified, heat inactivated, EU-approved, South America Origin | Thermo Fisher Scientific | Cat # 11550356 |
| FGF2 (Fibroblast Growth Factor) | PeproTech | Cat # 100-18B |
| FLT3 (FMS-like tyrosine kinase 3 receptor) ligand | PeproTech | Cat # 300-19 |
| GMAX (GlutaMAX™) 100× | Thermo Fisher Scientific | Cat # 35050-061 |
| Ham’s F12 | Thermo Fisher Scientific | Cat # 11765-062 |
| IGF2 (Insulin-like growth factor 2) | PeproTech | Cat # 100-12 |
| IL-3 (Interleukin 3) | PeproTech | Cat # 200-03 |
| IL-6 (Interleukin 6) | PeproTech | Cat # 200-06 |
| IMDM (Iscove's Modified Dulbecco's Media) with no phenol red | Themo Fisher Scientific | Cat # 21056-023 |
| ITS-E (Insulin-Transferrin-Selenium-E) | In Vitria | Cat # 777ITS092 |
| KOSR (KnockOutTM Serum Replacement) | Thermo Fisher Scientific | Cat # 10828028 |
| L-AA (L-Ascorbic acid) | Merck | Cat # A4403 |
| Linoleic acid | Merck | Cat # L2376 |
| Linolenic acid | Merck | Cat # L1376 |
| Matrigel® Growth Factor Reduced phenol red- free | In Vitro Technologies | Cat # FAL356231 |
| NaCl (Sodium chloride) | Merk | Cat # S5886 |
| NEAA (Non-essential amino acids) 100× | Thermo Fisher Scientific | Cat # 11140-050 |
| OsrHSA (Recombinant human serum albumin derived from Oryza sativa rice grain) | ScienCell Research Labs, Carlsbad, CA | Cat # OsrHSA |
| PBS (Phosphate Buffered Saline), no calcium, no magnesium | Thermo Fisher Scientific | Cat # 14190144 |
| Pen/Strep (Penicillin/Streptomycin) | Thermo Fisher Scientific | Cat # 15140122 |
| PFHMII (Protein Free Hybridoma Medium II) | Thermo Fisher Scientific | Cat # 12040077 |
| Polysorbate 80 (Tween 80) | Merck | Cat # P1754 |
| PVA (Polyvinyl alcohol) | Merck | Cat # P8136 |
| SB431542 | Sapphire Bioscience | Cat # 13031 |
| Soybean Oil / lecithin | Merck | Cat # S7381 |
| SCF (Stem cell factor) | PeproTech | Cat # 300-07 |
| SyntheChol | Merck | Cat # S5442 |
| TPO (Thrombopoietin) | PeproTech | Cat # 300-18 |
| TrypLETM Select dissociation Enzyme | Thermo Fisher Scientific | Cat # 12563011 |
| VEGF (Vascular endothelial growth factor) | PeproTech | Cat # 100-20 |
| Experimental Models: Cell Lines | ||
| SOX17mCHERRY/wRUNX1CGFP/w hESC H9 line | Ng et al., 2016 | N/A |
| Inducible RUNX1-ETO SOX17mCHERRY/wRUNX1CGFP/w hESC H9 line | Nafria et al., 2020 | N/A |
| Other | ||
| 18G needle × 1.5 inches, Terumo® AGANITM | Medisave | Cat # AN-1838R |
| 21G needle × 2 inches, Terumo® AGANITM | Medisave | Cat # AN-2150R |
| 23G needle × 1.25 inches, Terumo® AGANITM | Medisave | Cat # AN-2332R |
| 25G needle × 1 inch, Terumo® AGANITM | Medisave | Cat # AN-2525R |
| 5 mL round-bottom tubes for flow cytometry, Polypropylene, FalconTM REF 352002 | Thermo Fisher Scientific | Cat# 10519901 |
| 50 μm strainer filter, Sysmex CellTricksTM | Wolflabs | Cat # 04-004-2327 |
| 70 μm sterile strainer cap, Greiner Bio-One EASYstrainer | Kisker Biotech | Cat # 542070 |
| Low attachment round-bottomed 96-well plates (Sterile) | Costar | Cat # COR3788 |
| Sterile reagent reservoir for multichannel pipettes | StarLab | Cat # S4026-5806 |
| T25: Corning® cell culture flasks, surface area 25 cm2, canted neck, cap (vented) | Merck | Cat # 430639 |
| T75: Corning® cell culture flasks, surface area 75 cm2, canted neck, cap (vented) | Merck | Cat # 430641U |
| Tissue culture treated 6-well plates | Merck | Cat # CLS3516 |
| Vacuum Filtration system, Corning® 500 mL, 0.22 μm Pore 33.2 cm2 PES Membrane, Sterile | Merck | Cat # 431097 |
| Variable oxygen control CO2 incubator: Forma™ Series 3 Water Jacketed | Thermo Fisher Scientific | Cat # 4140TS |
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