Table 4:
Significant genes driving pathway enrichment for acetyl-CoA metabolic process in DINP females
| Gene | PPAR target gene based on expression | PPAR target gene based on ChIP-seq | No. of PPREs in TSS-flanking region | No. of ChIP-seq peaks | PND21 LFC | PND21 P-value | 10-month LFC | 10-month P-value |
|---|---|---|---|---|---|---|---|---|
| Fasn | PPARα, PPARδ/β | PPARα, PPARγ | 5 | 17 | 1.62 | 0.011* | 1.66 | 0.0074** |
| Acaca | NA | PPARγ | NA | 16 | 0.76 | 0.06^ | 1.53 | 0.0041** |
| Pdk4 | PPARα, PPARδ/β, PPARγ | PPARα, PPARα | 2 | 31 | 2.49 | 0.0011** | 1 | 0.053* |
| Pdha1 | PPARα | PPARα | 0 | 4 | 0.54 | 0.015* | 0.16 | 0.32 |
| Acacb | PPARα, PPARγ | PPARγ | 6 | 24 | 1.49 | 0.014* | 1.39 | 0.0015** |
| Acly | PPARδ/β | PPARα, PPARγ | 6 | 3 | 0.76 | 0.17 | 1.44 | 0.0091** |
| Mpc1 | NA | PPARδ, PPARβ | NA | 5 | −0.48 | 0.015* | −0.47 | 0.13 |
| Acss2 | PPARδ/β, PPARγ | PPARγ | 8 | 1 | 1.24 | 0.08^ | 1.31 | 0.027* |
| Mlycd | PPARα, PPARδ/β, PPARγ | PPARα, PPARγ | 2 | 9 | −0.25 | 0.13 | −0.45 | 0.0085** |
| Pdk2 | PPARγ | NA | 9 | NA | 0.11 | 0.55 | 0.75 | 0.005** |
| Cs | PPARγ | PPARγ | 3 | 5 | 0.76 | 0.0016** | 0.58 | 0.0099** |
| Mpc2 | NA | PPARα, PPARγ | NA | 6 | −0.26 | 0.29 | −0.9 | 0.01** |
| Dlat | PPARα, PPARγ | NA | 3 | 0 | 0.3 | 0.099^ | 0.55 | 0.0054** |
Genes were identified as PPAR target genes based on PPARgene.org, which utilizes a combination of evidence from published datasets and PPREs in regions flanking the TSS, and cistrome.org, which utilizes ChIP-seq data to identify transcription factor-binding sites in the promoter region. PND21 and 10-month log LFC and P-values (unadjusted) are from differential gene expression of RNA-seq data analyzed via edgeR QLF comparing DINP females to controls. Bolded genes were differentially expressed with an unadjusted P-value of ≤0.05 at both PND21 and 10 months.