Figure 4.

A summary of several DC-based vaccine strategies. A. The monocytes were obtained using CD14 beads isolated from blood. They then are cultured with cytokines to further derive functional DCs. After pulsing antigen these autologous DCs will be injected patient. B. There is another approach unlike described above. The Mo-DCs were obtained by extracorporeal photopheresis (ECP). C. The CD34⁺ isolated from UCB proliferating hematopoietic stem cells as a source of DC precursors have been developed. D. Employing a target antigen-fused anti-DC receptor antibody synthesize particles that targeted DCs in lymphoid tissues. The routes of administration include intranasal or by patch.