FIGURE 4.

Relative expression of cytokine genes in eqPBMC cultured in the presence of the G3 adjuvant, UV‐irradiated cyathostomin L3 or S. vulgaris L3, alone or combinations thereof. The effect of cyathostomin L3 or S. vulgaris L3 was evaluated in two separate experiments (closed and open circle, respectively). The cytokine gene expression was normalized to the geometric mean for the reference genes (SDHA and RPL32) and calibrated to that in the medium control. FC > 2 (indicated by dashed line) were considered as up‐regulated. Significant differences between stimulations are indicated by *<0.05, **<0.01, ***<0.001