Table 2.
Parameters for the Identification and Quantification of Dimethyl Labeled Peptides
| Parameters | Settings |
|---|---|
| Database | Yeast (Saccharomyces cerevisiae), canonical, UniProt, 6729 entries |
| Enzyme | Trypsin KR_C (Full specific) |
| Maximum number of missed cleavages | 3 |
| Precursor tolerance | 10 p.p.m. |
| Fragment tolerance | 20 p.p.m. |
| Dynamic modification | Dimethyl (Light, +28.0313 Da) Dimethyl (Heavy, +34.0631 Da) |
| Quantitation type | Labeling - SILAC etc |
| Multiplicity | 2 |
| FDR | <1% at peptides level |
| Peptide mass | [600, 10,000] |
| Peptide length | [6, 100] |
| Open search | FALSE |