REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Tris-HCl	Sigma-Aldrich	Cat# 10812846001
Acetonitrile with 0.1% Formic Acid (v/v), LC/MS Grade	Fisher Scientific	Cat# LS1201 CAS# 75-05-8
Water with 0.1% Formic Acid (v/v), LC/MS Grade	Fisher Scientific	Cat# LS1181 CAS# 7732-18-5
Formic Acid, 99.0+%, LC/MS Grade	Fisher Scientific	Cat# A11750 CAS# 64-18-6
Sequencing-grade modified Trypsin	Promega	Cat# V5111
Guanidine-HCl	Sigma-Aldrich	Cat# G3272
Iodoacetamide	Sigma-Aldrich	Cat# I6125
Ammonium Bicarbonate	Sigma-Aldrich	Cat# A6141
Software and Algorithms
MethylSight	Biggar et al., 2020	www.methylsight.com
Skyline	MacLean et al., 2010	https://www.skyline.ms/
ExPASy PeptideCutter	Gasteiger et al., 2003	https://web.expasy.org/peptide_cutter/