Chemicals, Peptides, and Recombinant Proteins |
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Yeast Nitrogen Base w/o AA, Carbohydrate & w/AS |
US biological |
Cat# Y2025 |
Drop-out Mix Complete w/o Yeast Nitrogen Base |
US biological |
Cat# D9515 |
D-glucose |
Sigma Aldrich |
Cat# 8270-10KG |
D-raffinose |
Bio-connect |
Cat# OR06197_2kg |
D-galactose |
Sigma Aldrich |
Cat# G0750-500G |
Yeast extract powder (YEP) |
VWR international |
Cat# 212750 |
Bacto-agar |
Becton Dickinson |
Cat# 214030 |
Agarose MP |
Sigma Aldrich |
Cat# 11388991001 |
Potassium Hydroxide |
Sigma Aldrich |
Cat# P5958-1KG |
Ethanol |
Honeywell |
Cat# 32221-2.5L |
Concanavalin A from Canavalia ensiformis (Jack bean) |
Sigma Aldrich |
Cat# L7647-250MG |
PBS tablets |
Gibco |
Cat# 17912-014 |
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Other |
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Cover glasses (#1.5, thickness 0.17 mm round, 25 mm) |
VWR |
Cat# 631-0172 |
Wash-N-Dry coverslip rack |
Sigma Aldrich |
Cat# Z688568-1EA |
400 mL tall glass beakers |
Novodirect |
Cat # 15439093 |
Whatman® Puradisc syringe filters (FP 30/0.2, pore size 0.2 μm) |
Sigma Aldrich |
Cat# Z612529 |
CELLView cell culture dish, PS, 35/10 mm, glass bottom |
Greiner Bio-One B.V. |
Cat# 627861 |
Attofluor™ Cell Chamber, for microscopy |
Thermofisher Scientific |
Cat# A7816 |