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. 2020 Oct 29;1(3):100150. doi: 10.1016/j.xpro.2020.100150

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Expected Outcomes

(A) Yield of a single assay. Shown are day 12 organoids distributed into 24 wells.

(B) Single 24-well of the same assay.

(C) High efficiency of tubule formation, shown are day 12 organoids.

(D) Confocal images of immunohistological stainings on sections of day 12 organoids (derived from the MANZ-2-2 iPSC line) showing WT1⁺ podocytes, EPCAM⁺ or HNF1B⁺ tubular epithelia, LRP2⁺ or CUBN⁺ proximal tubules, and CHD1⁺ distal tubules and connecting/collecting duct epithelia. Scale bars, 0.5 cm (B); 1 mm (C); 100 μm (D).