Figure 4.

Troubleshooting

(A) Low efficiency of tubule formation.

(B) Absence of tubule formation in small organoids.

(C) Occasional fusing of embryoid bodies at day 3 (asterisks); high magnification of fused embryoid bodies is shown in (C′).

(D) Large embryoid bodies due to clumping.

(E) Elimination of large embryoid bodies through a 500 μm strainer.

(F) Severe clumping of day 3 embryoid bodies.

(G) Areas of non-renal outgrowth (i.e., the uniformly colored areas lacking the outlines of tubules, arrows) in day 20 organoids.

Scale bars, 1 mm (C, D, and F); 400 μm (A, B, C′, and G).