REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies and Lectins
CALB1 rabbit (use 1:1,000). Labels distal tubules and collecting ducts	Sigma	Cat#C2724
CDH1 (E-Cadherin) mouse IgG2a (use 1:300). Labels distal tubules and collecting ducts	BD Biosciences	Cat#610181
CUBN rabbit IgG (use 1:500). Labels proximal tubules	Abcam	Cat#ab244274
EPCAM rabbit (use 1:100). Labels all tubular epithelia	Sigma	Cat#HPA026761
HNF1B rabbit (use 1:500). Labels nuclei of all tubular epithelia	Sigma	Cat#HPA002083
Lotus Tetragonolobus Lectin (LTL), Fluorescein (use 1:300). Labels proximal tubules	Vectorlabs	Cat#FL-1321
LRP2 (Megalin) mouse IgG1 (use 1:500). Labels proximal tubules	Novus Biologicals	Cat#NB110-96417
NPHS1 sheep IgG (use 1:200). Labels podocytes	R&D Systems	Cat#AF4269
SLC12A1 rabbit (use 1:1,000). Labels thick ascending limb	Sigma	Cat#HPA018107
WT1 mouse IgG1 (use 1:25). Labels podocyte nuclei	Santa Cruz Biotechnology	Cat#sc-7385
Hoechst 33258 (use 1:2,000/10 μM). Nuclear counterstain	Abcam	Cat#ab228550
Anti-rabbit-Dylight 488 (use 1:600)	Abcam	Cat#ab96899
Anti-mouse-Dylight 594 (use 1:600)	Abcam	Cat#ab96881
Anti-sheep-Alexa Fluor 594 (use 1:600)	Thermo Fisher	Cat#A11016
Chemicals, Peptides, and Recombinant Proteins
1-Thioglycerol	Sigma	Cat#M6145
2-Mercaptoethanol	Thermo Fisher	Cat#21985023
Accutase	STEMCELL Technologies	Cat#7920
Agar	Sigma	Cat#A1296
AlbumiNZ™ protease reduced, immunoassay (EIA) grade, ≥97% (BSA used for immunohistochemistry)	MP Biomedicals	Cat#219989880
Chemically Defined Lipid Concentrate	Thermo Fisher	Cat#11905031
CHIR99021	STEMCELL Technologies	Cat#72054
Dispase	STEMCELL Technologies	Cat#7923
DMEM, low glucose, pyruvate	Thermo Fisher	Cat#11885084
DPBS 1×, no calcium, no magnesium	Thermo Fisher	Cat#14190250
Ethanol absolute	Sigma	Cat#1.07017
Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix	Thermo Fisher	Cat#A1413202
Gentle Cell Dissociation Reagent	STEMCELL Technologies	Cat#7174
GlutaMAX Supplement	Thermo Fisher	Cat#35050061
Ham's F12 Nutrient Mix	Thermo Fisher	Cat#11765054
HEPES	Thermo Fisher	Cat#15630080
Horse serum	Sigma	Cat#H1270
HyAgarose™ LE Agarose	HydraGene	Cat#R9012LE
Hydrochloric acid	Sigma	Cat#320331
IMDM	Thermo Fisher	Cat#12440053
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X)	Thermo Fisher	Cat#51500056
K-252a (BDNF inhibitor)	Sigma	Cat#K1639
KnockOut™ Serum Replacement - Multi-Species	Thermo Fisher	Cat#A3181502
L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate	Sigma	Cat#A8960
Leica Surgipath Paraplast (Paraffin)	bio-strategy	Cat#LEIS39601006
Matrigel® Growth Factor Reduced Basement Membrane Matrix, LDEV-free	BD Biosciences	Cat#354230
MEM Non-Essential Amino Acids Solution	Thermo Fisher	Cat#11140050
mTeSR1	STEMCELL Technologies	Cat#5850
Paraformaldehyde 8%	Emgrid Australia	Cat#157-8
Penicillin-Streptomycin	Thermo Fisher	Cat#15140122
PFHM-II Protein-Free Hybridoma Medium	Thermo Fisher	Cat#12040077
Plasmocin	InvivoGen	Cat#ant-mpt
Poly(vinyl alcohol)	Sigma	Cat#P8136
Probumin® Bovine Serum Albumin	Sigma	Cat#821006
ProLong™ Diamond Antifade Mountant	Thermo Fisher	Cat#P36961
ROCK inhibitor Y-27632	STEMCELL Technologies	Cat#72304
Sodium chloride	Sigma	Cat#71376
Trisodium citrate dihydrate	Sigma	Cat#W302600
StemFlex™ Medium	Thermo Fisher	Cat#A3349401
Sucrose	Sigma	Cat#S0389
TeSR™-E8™	STEMCELL Technologies	Cat#05990
Tissue-Plus™ OCT Compound	Fisher Scientific	Cat#23-730-571
Triton X-100	Sigma	Cat#X100
TRIzol™ Reagent	Thermo Fisher	Cat#15596026
Tween 20	Sigma	Cat#P1379
UltraPure™ Tris Buffer	Thermo Fisher	Cat#15504020
Xylene	Sigma	Cat#534056
Other
Cell lifter	Corning	Cat#3008
Corning tissue-culture treated culture dishes, D × H 100 mm × 20 mm	Merck Millipore	Cat#430167
Corning disposable spinner flasks	Sigma	Cat#CLS3152
Fisherbrand™ Multi Function 3D rotator	Fisher Scientific	Cat#15514080
Fisherbrand™ Histosette™ II Tissue Processing/Embedding Cassettes	Fisher Scientific	Cat#15-182-708A
ImmEdge® Hydrophobic Barrier PAP Pen	Vector Labs	Cat#H-4000
LockMailer™ - Microscope Mailer and Staining Jar	Ted Pella	Cat#21096
Menzel™ Microscope Coverslips	Fisher Scientific	Cat#11778691
Nunc® CryoTubes® 1.8 mL, Internal thread	Sigma	Cat#V7634
pluriStrainer® 500 μm	Pluri Select	Cat#43-50500-03
Stericup-GP Sterile Vacuum Filtration System (500 mL)	Merck Millipore	Cat#S2GPU05RE
Stericup-GP Sterile Vacuum Filtration System (200 mL)	Merck Millipore	Cat#SCGPU02RE
Superfrost Plus Microscope Slides	Thermo Fisher	Cat#4951PLUS4
Tissue-Tek® Cryomold	VWR	Cat#4565
Ultra-Low Attachment 24-well plates	Merck Millipore	Cat#CLS3473
Ultra-Low Attachment 6-well plates	Merck Millipore	Cat#CLS3471
Oligonucleotides
Primers for NPHS1 (marker for podocytes), fwd: AGTGTGGCTAAGGGATTACCC; rev: TCACCGTGAATGTTCTGTTCC	Przepiorski et al., 2018	n/a
Primers for LRP2 (marker for proximal tubule), fwd: AAATTGAGCACAGCACCTTTGA; rev: TCTGCTTTCCTGACTCGAATAATG	Przepiorski et al., 2018	n/a
Primers for SLC34A1 (marker for proximal tubule), fwd: CCATCATCGTCAGCATGGTCT; rev: GACAGCCAGTTAAAGCAGTCA	This paper	n/a
Primers for SLC12A1 (marker for thick ascending limb), fwd: AGTGCCCAGTAATACCAATCGC; rev: GCCTAAAGCTGATTCTGAGTCTT	Przepiorski et al., 2018	n/a
Primers for UMOD (marker for thick ascending limb), fwd: CGGCGGCTACTACGTCTAC; rev: TGCCATCTGCCATTATTCGATTT	Przepiorski et al., 2018	n/a
Primers for SLC12A3 (marker for distal tubule), fwd: CCTGGGTGGAGACCTTCATTC; rev: GAGCCCCAATTTACCTCTGGC	This paper	n/a
Primers for CDH1 (marker for distal tubule and collecting duct), fwd: ATTTTTCCCTCGACACCCGAT; rev: TCCCAGGCGTAGACCAAGA	Przepiorski et al., 2018	n/a
Primers for CALB1 (marker for distal tubule and collecting duct), fwd: TCCAGGGAATCAAAATGTGTGG; rev: GCACAGATCCTTCAGTAAAGCA	Przepiorski et al., 2018	n/a
Primers for GATA3 (marker for distal tubule and collecting duct), fwd: GCCCCTCATTAAGCCCAAG; rev: TTGTGGTGGTCTGACAGTTCG	Przepiorski et al., 2018	n/a
Primers for HPRT1 (reference housekeeping gene), fwd: CATTATGCTGAGGATTTGGAAAGG; rev: CTTGAGCACACAGAGGGCTACA	Przepiorski et al., 2018	n/a
Experimental Models: Cell Lines
MAFB-P2A-eGFP H9 (ESC, female, labels podocytes)	Tran et al., 2019	n/a
HPSI0214i-wibj_2 (iPSC, female)	HipSci	n/a
CRL1502 (clone C32) (iPSC, female)	n/a	RBK/GUDMAP Resources
LRP2:mTagBFP2 (iPSC, male, labels proximal tubules)	Howden et al., 2018; Vanslambrouck et al., 2019	RBK/GUDMAP Resources
BJ RiPS (iPSC, male)	Warren et al., 2010; Przepiorski et al., 2018	n/a
MANZ-2-2 (iPSC, female)	Przepiorski et al., 2018; Oh et al., 2020.	n/a
MANZ-4-37 (iPSC, male)	Przepiorski et al., 2018; Oh et al., 2020.	n/a
HNF1B-/-, 3 clones (iPSC, female, isogenic to MANZ-2-2)	Przepiorski et al., 2018	n/a
CTNS-mutant lines, 3 clones (iPSC, male, patient-derived)	Hollywood et al., 2020	n/a
CTNS knockout lines, 3 clones (iPSC, female, isogenic to CRL1502 (clone C32))	Hollywood et al., 2020	n/a