LC Solvent A	Final Concentration	Amount
Formic Acid	0.1% (v/v)	1 mL
MS H2O		999 mL
Total		1,000 mL

LC Solvent B	Final Concentration	Amount
Formic Acid	0.1% (v/v)	1 mL
Acetonitrile	80% (v/v)	800 mL
MS H2O		199 mL
Total		1,000 mL

Ammonium Bicarbonate (ABC) (pH ~8.0)	Final Concentration	Amount
ABC	100 mM	79.06 mg
MS H2O		10 mL
Total		10 mL

Iodoacetamide (IAA)	Final Concentration	Amount
IAA	375 mM	34.68 mg
MS H2O		0.5 mL
Total		0.5 mL

Dithiothreitol (DTT)	Final Concentration	Amount
DTT	60 mM	46.28 mg
MS H2O		5 mL
Total		5 mL

MS-grade Trypsin/Lys-C Mix Stock	Final Concentration	Amount
Trypsin/LysC mix	1 μg/μL	20 μg
MS H2O		20 μL
Total		20 μL

RIPA Buffer with 1× Halt Protease Inhibitor	Final Concentration	Amount
Halt protease inhibitor cocktail (100×)	1×	10 μL
MS H2O		990 μL
Total		1 mL