REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
10× HBSS	Fisher Scientific	MT-200-23CV
Bovine serum albumin	Sigma-Aldrich	A8806
Collagenase type XI	Sigma-Aldrich	C7657
RPMI 1640	Fisher Scientific	MT10040CV
Histopaque-1077	Sigma-Aldrich	10771
Phosphate-buffered saline	Sigma-Aldrich	D8537
Accutase	Innovative Cell Technologies	AT-104
Antibiotic-antimycotic solution	Fisher Scientific	MT30004CI
Fetal bovine serum	Fisher Scientific	F0926
Trypan blue	VWR	97063-702
Critical Commercial Assays
Chromium Single Cell 3′ Library & Gel Bead Kit v2	10X Genomics	PN-120237
Deposited Data
scRNA-seq data	GEO	GSE144471
Experimental Models: Organisms/Strains
Mouse: NOD/ShiLtJ (5 weeks)	Jackson Laboratory	001976
Other
5.5 inch surgical scissors	Roboz	RS-6782
3.5 inch small curved surgical scissors	Roboz	RS-5911
2 inch curved bulldog clamp	Roboz	RS-7439
4 inch tissue forceps	Roboz	RS-5153
30G needle	VWR	76290-488
5 mL syringe	BD	309647
50 mL conical tubes	Fisher Scientific	12-565-270
15 mL conical tubes	Fisher Scientific	12-565-268
40 μm cell strainer	Fisher Scientific	087711
Kimwipes	Kimberly-Clark	34155
6 W LED Dual Goose-neck Illuminator	Amscope	LED-6W
20× to 40× stereo microscope with bottom lighting	Amscope	SE306R-P-LED
3.5× to 90× Zoom Trinocular Stereo Microscope with Table Pillar Stand	Amscope	SM-1TSZ-V203
1,000 μm Spectra Mesh Woven Filters, cut into 2 inch squares	Fisher Scientific	08-670-183
Water bath	Fisher Scientific	n/a
Refrigerated centrifuge	Fisher Scientific	n/a
Countess II automated cell counter	Fisher Scientific	AMQAX1000