Table 5.

Diluting Prepared Samples Collected from Transfection

	1:10,000 Dilution	1:50,000 Dilution	1:250,000 Dilution
Concentrated Vector 1:100	Tube 1	Tube 2	Tube 3
	495 μL SD solution	400 μL SD solution	400 μL SD solution
	5 μL diluted sample	100 μL from Tube 1	100 μL from Tube 2
Non-concentrated Vector 1:10	Tube 1a	Tube 2a	\
	490 μL SD solution	400 μL SD solution	\
	10 μL diluted sample	100 μL from Tube 1a	\