Table 2.
Marker | Fluorochrome | Comp Bead | 1X μL/test | 10X (+1) μL/test | 20X (+3) μL/test |
---|---|---|---|---|---|
CXCR4 | PE Cy5 | mouse | 2.5 | 30 | 57.5 |
CXCR3 | AF700 | mouse | 2 | 24 | 46 |
CCR7 | APC-Cy7 | mouse | 2 | 24 | 46 |
CCR6 | BV421 | mouse | 1 | 12 | 23 |
CCR4 | PE-CF594 | mouse | 1 | 12 | 23 |
CCR5 | BV650 | mouse | 0.6 | 7.2 | 13.8 |
Sum of antibodies | 9.1 | 109.2 | 209.3 | ||
PBS | 35.9 | 430.8 | 825.7 | ||
Final volume | 45 | 540 | 1,035 |