Table 4.
Extracellular Antibody Cocktail with CLA
| Marker | Fluorochrome | Comp Bead | 1X μL/test | 10X (+1) μL/test | 20X (+3) μL/test |
|---|---|---|---|---|---|
| CD69 | PE | mouse | 10 | 110 | 230 |
| CD27 | BV570 | mouse | 2 | 22 | 46 |
| CD127 | BV480 | mouse | 2 | 22 | 46 |
| PD-1 | BV785 | mouse | 2 | 22 | 46 |
| CD38 | BUV661 | mouse | 1.5 | 16.5 | 34.5 |
| CD95 | BV711 | mouse | 1 | 11 | 23 |
| CXCR5 | BB515 | rat | 0.7 | 7.7 | 16.1 |
| CD4 | BB700 | mouse | 0.6 | 6.6 | 13.8 |
| CLA | AF647 | mouse | 0.5 | 5.5 | 11.5 |
| CD19 | BV510 | mouse | 0.5 | 5.5 | 11.5 |
| CD14 | BV510 | mouse | 0.5 | 5.5 | 11.5 |
| CD45RA | BUV563 | mouse | 0.5 | 5.5 | 11.5 |
| CD25 | BUV737 | mouse | 0.5 | 5.5 | 11.5 |
| CD8 | BUV496 | mouse | 0.5 | 5.5 | 11.5 |
| CD3 | BUV805 | mouse | 0.5 | 5.5 | 11.5 |
| HLA-DR | BV605 | mouse | 0.4 | 4.4 | 9.2 |
| Sum of antibodies | 23.7 | 260.7 | 545.1 | ||
| Staining buffer | 26.3 | 289.3 | 604.9 | ||
| Final volume | 50 | 550 | 1,150 | ||