. 2020 Oct 29;1(3):100154. doi: 10.1016/j.xpro.2020.100154

Table 7.

Flow Cytometry Panel

Laser	Filter	Fluorochrome	Antigen	Manufacturer	Clone	Dilution^a
488 nm	515/20	BB515	CXCR5	BD	RF8B2	140
488 nm	710/50	BB700	CD4	BD	SK3	160
640 nm	660/20	AF647	CLA^b	Biolegend	HECA-452	200
	660/20	AF647	SLe^X (CD15S)^b	BD	CSLEX1	200
	730/45	AF700	CXCR3	Biolegend	G025H7	50
	780/60	APC-Cy7	CCR7	Biolegend	G043H7	50
355 nm	379/28	BUV395	Ki67	BD	B56	200
	515/30	BUV496	CD8	BD	RPA-T8	200
	586/15	BUV563	CD45RA	BD	HI100	200
	660/20	BUV661	CD38	BD	HIT2	66.7
	740/35	BUV737	CD25	BD	2A3	200
	820/60	BUV805	CD3	BD	UCHT1	200
405 nm	450/50	BV421	CCR6	BD	11A9	100
	470/15	BV480	CD127	BD	HIL-7R-M21	50
	515/20	Aqua Blue	Viability	Invitrogen	n/a	20
	515/20	BV510	CD14	Biolegend	M5E2	200
	515/20	BV510	CD19	Biolegend	HIB19	200
	586/15	BV570	CD27	Biolegend	O323	50
	610/20	BV605	HLA-DR	BD	G46-6	100
	660/20	BV650	CCR5	BD	3A9	166.7
	710/50	BV711	CD95	Biolegend	DX2	100
	780/60	BV785	PD-1	Biolegend	EH12.2H7	50
561 nm	586/15	PE	CD69	BD	FN50	10
	610/20	PE-CF594	CCR4	BD	1G1	100
	660/40	PE-Cy5	CXCR4	BD	12G5	40
	780/60	PE-Cy7	FoxP3	eBioscience	236A/E7	20

n/a, not applicable; BB, brilliant blue; AF, Alexa Fluor; APC, allophycocyanin; BUV; Brilliant Ultraviolet; BV, Brilliant Violet; PE, phycoerythrin.

Dilutions are calculated by titration of all antibodies. In some cases, antibody volumes/dilution factors are rounded up to facilitate pipetting (for example, CD38 BUV661 is used at a 1.5 μL/test or a 1:66.7 dilution).

This protocol indicates the staining of CLA AF647 and SLe^X AF647 in two independent panels, not simultaneously in the same antibody cocktail.