Table 2.

Settings for 1st PCR. Rows in gray are performed for 15 cycles.

PCR Cycling Conditions
Steps	in °C	Time	Cycles
Initial Denaturation	98	30 s	1
Denaturation	98	5 s	15
Annealing	55	10 s
Extension	72	10 s
Final Extension	72	2 min	1
Hold	10	∞