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Master Mix for 2nd PCR
| Reagents | Volume in μL/Sample |
|---|---|
| Phusion® HF Buffer (without Dye) | 10 |
| dNTPs (20 μmol, Bioline BIO-39043) | 1 |
| Forward primer (e.g., 341-ovh-HTS- SC501 Primer (20 μM)) | 0.313 |
| Phusion® High-Fidelity DNA Polymerase Hotstart | 0.2 |
| DMSO (100%) | 1.5 |
| Water (for molecular biology, DEPC-treated and filter-sterilized) | 32.487 |
