Table 5.

Settings for 2nd PCR. Rows in gray are performed for ten cycles.

PCR Cycling Conditions
Steps	Temperature	Time	Cycles
Initial Denaturation	98	30 s	1
Denaturation	98	5 s	10
Annealing	55	10 s
Extension	72	10 s
Final Extension	72	2 min	1
Hold	10	∞