Table 1.

Percentage of mCherry-, YFP-, and Double-Positive Cells Induced by BATTLE-1 with Different Ratios of Cre and FLPO

	mCherry-Positive Cells (%) (Mean ± SEM)	YFP-Positive Cells (%) (Mean ± SEM)	mCherry, YFP− Double-Positive Cells (%) (Mean ± SEM)	Viral Titers of BATTLE-1 Lentiviruses	Total Cell and Mouse Numbers
Cre:FLPO 1:1	61.3 ± 5.8	38 ± 5.8	0.7 ± 0.2	5 × 108 copies each	n = 827, N = 5
Cre:FLPO 10:1	92 ± 1.4	7.4 ± 1.6	0.6 ± 0.4	5 × 108, 5 × 107 copies each	n = 876 N = 3
Cre:FLPO 100:1	98.7 ± 0.2	1.1 ± 0.2	0.2 ± 0.1	5 × 108, 5 × 106 copies each	N = 1,153, N = 3

The BATTLE-1 method mostly induced split-tunable expression of mCherry and YFP in dentate granule neurons in the hippocampus. The data of Cre:FLPO = 1:1, and Cre:FLPO= 1:0.01 were adopted from Figures 1D and 1G in Kohara et al., 2020.