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| Digestion Buffer | Final Concentration | Amount |
|---|---|---|
| Tris-HCl pH8 (1 M) | 50 mM | 0.5 mL |
| EDTA (0.5 M) | 1 mM | 0.02 mL |
| Guanidine Hydrochloride (8 M) | 0.8 M | 1 mL |
| Triton X-100 (10%) | 0.5% | 0.5 mL |
| ddH2O | n/a | 7.88 mL |
| Proteinase K (800 units/mL) | 8 units/mL | 0.1 mL |
| Total | n/a | 10 mL |
Store digestion buffer (without Proteinase K) as aliquots at −20°C for a few months. Add Proteinase K right before use.
