REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Collagen I | Abcam | Cat#ab34710; RRID:AB_731684 |
αSMA | Sigma-Aldrich | Cat#C6198; RRID:AB_476856 |
Alexa Fluor 488-conjugated donkey anti-rabbit | Jackson ImmunoResearch | Cat#711-545-152; RRID:AB_2313584 |
Alexa Fluor 647-conjugated donkey anti-mouse | Jackson ImmunoResearch | Cat#715-605-151; RRID:AB_2340863 |
Other | ||
Cryostat, CM3050S | Leica | N/A |
Vibratome, VT1200S | Leica | N/A |
CellHesion200 | Bruker | N/A |
Fluorescent microscope, Nikon Ti | Nikon | N/A |
Chemicals, Peptides, and Recombinant Proteins | ||
Mayer’s hemalum solution (for H&E staining) | Millipore/Sigma-Aldrich | Cat#1.09249.0500 |
Eosin Y (for H&E staining) | Millipore/Sigma-Aldrich | Cat#1.15935.0100 |
Dulbecco’s phosphate-buffered saline (D-PBS) | Gibco | Cat#14040133 |
DAPI | Invitrogen | Cat#D1306; RRID:AB_2629482 |
OCT Compound | Tissue-Tek | Cat#4583 |
Triton X-100 | Sigma | Cat#T8787 |
cOmplete Mini EDTA-free | Roche | Cat#11836170001 |
Cryomold (15 mm × 15 mm × 5 mm) | Tissue-Tek | Cat#4566 |
Cover Slips, Circles, diameter 10 mm | Thermo Scientific | Cat#12893804 |
Poly-L-lysine solution | Sigma-Aldrich | Cat#P4707 |
Flat-tipped cantilever, MLCT | Bruker | N/A |
Silica bead, 10 μm | Microparticles | SiO2-F-SC100 |
Agarose, low gelling temperature | Sigma | A9414 |
Commercial cantilever with 10 μm spherical tip | Novascan Tech | PT.GS |
Commercial cantilever with 10 μm spherical tip | NanoAndMore | Sqube |
2-component epoxy glue | Araldite, Switzerland | Rapid |
2-component epoxy glue | UHU, Germany | 3996 |
Glass bottom dishes, CellHesion 200 compatible, FluoroDish Cell Culture Dishm 35 mm, 23 mm well | WPI | FD35-100 |
Pluronic | Sigma | P2443 |
Software and Algorithms | ||
NIH ImageJ | NIH | N/A |
R | N/A | https://www.r-project.org |
Pheatmap function | Raivo Kolde | N/A |
CellHesion 200 | JPK | N/A |
Prism 7.0 software | GraphPad | N/A |