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. 2020 Nov 16;1(3):100167. doi: 10.1016/j.xpro.2020.100167
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Collagen I Abcam Cat#ab34710; RRID:AB_731684
αSMA Sigma-Aldrich Cat#C6198;
RRID:AB_476856
Alexa Fluor 488-conjugated donkey anti-rabbit Jackson ImmunoResearch Cat#711-545-152;
RRID:AB_2313584
Alexa Fluor 647-conjugated donkey anti-mouse Jackson ImmunoResearch Cat#715-605-151;
RRID:AB_2340863

Other

Cryostat, CM3050S Leica N/A
Vibratome, VT1200S Leica N/A
CellHesion200 Bruker N/A
Fluorescent microscope, Nikon Ti Nikon N/A

Chemicals, Peptides, and Recombinant Proteins

Mayer’s hemalum solution (for H&E staining) Millipore/Sigma-Aldrich Cat#1.09249.0500
Eosin Y (for H&E staining) Millipore/Sigma-Aldrich Cat#1.15935.0100
Dulbecco’s phosphate-buffered saline (D-PBS) Gibco Cat#14040133
DAPI Invitrogen Cat#D1306;
RRID:AB_2629482
OCT Compound Tissue-Tek Cat#4583
Triton X-100 Sigma Cat#T8787
cOmplete Mini EDTA-free Roche Cat#11836170001
Cryomold (15 mm × 15 mm × 5 mm) Tissue-Tek Cat#4566
Cover Slips, Circles, diameter 10 mm Thermo Scientific Cat#12893804
Poly-L-lysine solution Sigma-Aldrich Cat#P4707
Flat-tipped cantilever, MLCT Bruker N/A
Silica bead, 10 μm Microparticles SiO2-F-SC100
Agarose, low gelling temperature Sigma A9414
Commercial cantilever with 10 μm spherical tip Novascan Tech PT.GS
Commercial cantilever with 10 μm spherical tip NanoAndMore Sqube
2-component epoxy glue Araldite, Switzerland Rapid
2-component epoxy glue UHU, Germany 3996
Glass bottom dishes, CellHesion 200 compatible, FluoroDish Cell Culture Dishm 35 mm, 23 mm well WPI FD35-100
Pluronic Sigma P2443

Software and Algorithms

NIH ImageJ NIH N/A
R N/A https://www.r-project.org
Pheatmap function Raivo Kolde N/A
CellHesion 200 JPK N/A
Prism 7.0 software GraphPad N/A