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Immunofluorescent (IF) staining of the fresh‐frozen sections. Both mouse liver and mouse pancreas sections were IF stained with αSMA (green), CK‐8 (red) (Panel A, scale bar: 250 µm), as well as B220 (red) and CD3 (green) (Panel B, scale bar: 250 µm) antibodies. Images obtained indicated positive staining for all antibodies across all three freezing techniques.
