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. 2020 Nov 25;1(3):100173. doi: 10.1016/j.xpro.2020.100173

Figure 1.

Figure 1

Design of the pIRO Construct

Schematic representation of the pIRO (pTM/Δ 5′SLAB–3′WT-Ribozyme) expression plasmid. The pIRO construct is composed of the T7 RNA polymerase promoter (blue box), a short part of the capsid coding region containing the 5′ cyclization sequence (CS), the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) to facilitate high-level RNA translation, the NS1 to NS5 coding region, the 3′ non-translated region (NTR), and the HDV ribozyme for generating authentic 3′ ends. The nucleotide positions of the ZIKV H/PF/2013 (GenBank accession number KJ776791.2) and DENV-2 genome (GenBank accession number NC_001474.2) used to generate the constructs are given below each element in brackets. Sequences of ZIKV are given in green; sequences corresponding to DENV are given in purple.