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. 2020 Nov 25;1(3):100184. doi: 10.1016/j.xpro.2020.100184

Figure 4.

Figure 4

Versatility of “Dark” Proxy-MADR Cells

(A) Schematic showing competence among pDonors to integrate their cassettes through dRMCE into the Rosa26 “PuroR” locus.

(B) Neuro-2a Rosa26 “dark-PuroR” Proxy-MADR cells expressing tdTomato, smFP-Bright, or TagBFP2 after dRMCE.

(C) Schematic showing integration of the Green-Red-FUCCI cassette through dRMCE into the Rosa26 “PuroR” locus.

(D) Neuro-2a Rosa26 “Green-Red FUCCI” Proxy-MADR cells expressing mVenus and mCherry after dRMCE and subsequent purification. Scale bar, 50 μm.