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Neuronal Maturation Medium
| Reagent | Final Concentration | Volume (μL or mL) |
|---|---|---|
| Neuronal Basal Media | N/A | 200 mL |
| BDNF (40 μg/mL) | 40 ng/mL | 200 μL |
| GDNF (40 μg/mL) | 40 ng/mL | 200 μL |
| Laminin (1.2 mg/mL) | 1 μg/mL | 167 μL |
| cAMP (500 mM) | 250 μM | 100 μL |
| Ascorbic Acid (200 mM) | 200 μM | 200 μL |
| PD0325901 (20 mM) | 10 μM | 100 μL |
| SU5402 (20 mM) | 10 μM | 100 μL |
| DAPT (20 mM) | 10 μM | 100 μL |
| ROCK Inhibitor (Y27632; 10 mM)a | 10 μM | 200 μL |
| Total | N/A | 200 mL |
ROCK inhibitor is added only on d15 and omitted on the following days
