Skip to main content
. 2020 Dec 22;8(2):e000725. doi: 10.1136/jitc-2020-000725

Figure 5.

Figure 5

Lean and DIO AdTR/CpG/PD-1 responders share a conserved tumoral gene expression profile that is distinct from those of non-responders and treatment-naive mice. (A) Heatmap depicting unbiased hierarchical clustering and gene expression patterns for 750 immune-related genes in the tumors of therapy-naive and therapy-treated lean and DIO animals. (B) Volcano plots showing expression of 750 genes in (left) lean and (right) DIO responders versus respective non-responders. Dashed line indicates an exploratory unadjusted p value threshold (p=0.05) to screen differentially expressed (DE) target genes. Dot colors correspond to genes from green, blue and yellow hierarchical clusters in (A) that were also significantly (p<0.05) DE in both lean and DIO responders versus non-responders. (C) NanoString-generated cell type scores comparing relative gene expression-based population abundance in responding and non-responding lean and DIO therapy-treated animals. DC, dendritic cell; DE, differentially expressed; DIO, diet-induced obese; no therapy, NT; non-responder, NR; PD-1, programmed cell death-1; Res, responder.