FIG. 2.

High glucose potentiated HOG-LDL-induced RPE barrier breakdown. Quiescent monolayer ARPE-19 cells were pretreated with normal glucose (5.5 mM), high glucose (25 mM), or mannitol (at a comparable concentration as an osmotic control) for 24 h, and then treated with N-LDL (200 mg/L) or HOG-LDL (100 or 200 mg/L) for 6 h. PBS in normal glucose served as the baseline control. (A) TEER and (B) FITC-dextran leakage were measured. Data are presented as percentages relative to the baseline control (mean ± SD, n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 versus N-LDL. ^#P < 0.05 versus the normal glucose or mannitol control.