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. 2020 Dec 1;1(3):100190. doi: 10.1016/j.xpro.2020.100190

Figure 4.

Figure 4

Timeline of Tri-culture Assembly and Representative Images of iMicroglia and Tri-culture

(A) Timeline for assembling the 30-day tri-culture.

(B) Representative image of D11 90 iMicroglia in mono-culture. Scale bar, 50 μm.

(C) Bright-field image of tri-culture at D30. White arrowhead identifying an iAstrocyte. Red arrowhead identifying an iMicroglia. Yellow arrowhead identifying an iNeuron. Scale bar, 50 μm.

(D) 3D reconstruction of IBA1+ (green) in contact with MAP2+ (yellow) dendrites that have SYN+ (red) synapses in a day 30 tri-culture. Scale bar, 20 μm.