Timeline of Tri-culture Assembly and Representative Images of iMicroglia and Tri-culture
(A) Timeline for assembling the 30-day tri-culture.
(B) Representative image of D11 90 iMicroglia in mono-culture. Scale bar, 50 μm.
(C) Bright-field image of tri-culture at D30. White arrowhead identifying an iAstrocyte. Red arrowhead identifying an iMicroglia. Yellow arrowhead identifying an iNeuron. Scale bar, 50 μm.
(D) 3D reconstruction of IBA1+ (green) in contact with MAP2+ (yellow) dendrites that have SYN+ (red) synapses in a day 30 tri-culture. Scale bar, 20 μm.