Reagent	Stock Concentration	Final Concentration	Volume
Base N2 medium (store at 4°C)
DMEM:F-12	n/a	n/a	490.5 mL
Glucose	n/a	0.75 g/500 mL	n/a
β-Mercaptoethanol	55 mM	55 μM	500 μL
Primocin	n/a	1:500	1 mL
N2 supplement B (add after filtration step with .22um CA filter)	n/a	1:100	5 mL
Total			50 0mL
N2 Medium + Small molecules (store at 4°C)
Base N2 medium	n/a	n/a	100 mL
BDNF	100 μg/mL	10 ng/mL	10 μL
NT3	100 μg/mL	10 ng/mL	10 μL
Laminin	1 mg/mL	200 ng/mL	20 μL
Doxycycline	100 mg/mL	2 μg/mL	2 μL
Total			100 mL
Sodium/pyruvate mixture for iN media (store at 4°C)
Neurobasal-A	n/a	n/a	20 mL
Glucose	n/a	500 mM	1.8 g
Sodium pyruvate	n/a	1 M	2.21 g
Total	n/a	n/a	20 mL
iN media for maintenance (store at 4°C)
Neurobasal-A	n/a	n/a	97 mL
B27 supplement with vitamin A	50×	1×	2 mL
Glutamax	100×	1×	1 mL
Glucose/sodium pyruvate mixture (100×)	100×	5 mM glucose/10 mM sodium pyruvate	1 mL
BDNF	100 μg/mL	10 ng/mL	10 μL
NT3	100 μg/mL	10 ng/mL	10 μL
Doxycycline	100 mg/mL	2 μg/mL	2 μL
Total	n/a	n/a	100 mL
High FGF Astrocyte media for first stage maintenance (store at 4°C)
N2 base media	n/a	n/a	88mL
FBS	n/a	10%	10mL
B27 with vitamin A	50×	1×	2mL
FGF-2	20 μg/mL	20 ng/mL	100 μL
EGF	20 μg/mL	20 ng/mL	100 μL
Total	n/a	n/a	100 mL
Low FGF Astrocyte media for second stage maintenance (store at 4°C)
N2 base media	n/a	n/a	88 mL
FBS	n/a	10%	10 mL
B27 with vitamin A	50×	1×	2 mL
FGF-2	20 μg/mL	5 ng/mL	25 μL
Total	n/a	n/a	100 mL
Base RPMI media for CMP differentiation (store at 4°C)
Corning 500 mL RPMI 1640	n/a	n/a	97.6 mL
Glutamine	200 mM	2 mM	1 mL
Penicillin/streptomycin	n/a	1:1,000	100 μL
MTG	n/a	400 μM	300 μL
Ascorbic acid	5 mg/mL	50 μg/mL	1 mL
Total	n/a	n/a	100 mL
Base SP34 media for CMP differentiation (store at 4°C)
StemPro-34 SFM (1×)	n/a	n/a	97.6 mL
Glutamine	200 mM	2 mM	1 mL
Penicillin/streptomycin	n/a	1:1,000	100 μL
MTG	n/a	400 μM	300 μL
Ascorbic acid	5 mg/mL	50 μg/mL	1 mL
Total	n/a	n/a	100 mL
Base SFD media for CMP differentiation (store at 4°C)
IMDM	n/a	75%	75 mL
HAMS F-12	n/a	25%	25 mL
N2-supplement	n/a	1:200	500 μL
B27 supplement w/0 retinoic acid	n/a	1:100	1 mL
10% BSA in PBS	n/a	1:200	500 μL
Glutamine	200 mM	2 mM	1 mL
Penicillin/streptomycin	n/a	1:1,000	100 μL
MTG	n/a	400 μM	300 μL
Ascorbic acid	5 mg/mL	50 μg/mL	1 mL
Total	n/a	n/a	104.4 mL
iMg media for differentiation and maintenance (store at 4°C)
HyClone RPMI 1640 media	n/a	n/a	45 mL
Characterized Fetal Bovine Serum, US Origin	n/a	10%	5 mL
Recombinant Human IL-34 Protein	100 μg/mL	100 ng/mL	50 μL
M-CSF Recombinant Human Protein	25 μg/mL	25 ng/mL	50 μL
Recombinant Human TGF-β1	50 μg/mL	50 ng/mL	50 μL
Pen/Strep	n/a	1:1,000	50 μL
Total	n/a	n/a	50 mL