REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CX3CR1 (rabbit) (1:500)	Abcam	ab8021
Glutamine synthetase (mouse) (1:500)	Millipore	MAB302
IBA1 (rabbit) (1:500)	WAKO	019-19741
MAP2 (chicken) (1:500)	Abcam	ab5392
P2RY12 (rabbit) (1:100)	Alomone Labs	APR-020
PSD95 clone K28/43 (mouse) (1:500)	NeuroMab	75-028
SOX9 (rabbit) (1:250)	Abcam	ab185230
Synaptophysin clone SY38 (mouse) (1:250)	Millipore	MAB5258
Thrombospondin-1 (rabbit) (1:250)	Abcam	ab85762
TMEM119 (rabbit)	Abcam	ab185333
Bacterial and Virus Strains
VSVG.HIV.SIN.cPPT.CMV.mNgn2.WPR	Wernig lab, Stanford University	received directly from Wernig lab (Addgene #52047)
VSVG.HIV.SIN.cPPT.CMV.rTTA.WPRE	Wernig lab, Stanford University	received directly from Wernig lab (Addgene alternative #19780)
Chemicals, Peptides, and Recombinant Proteins
Ascorbic acid	Sigma-Aldrich	A92902
Astrocyte media	ScienCell	1801
B-27 supplement	Thermo Fisher Scientific	17504044
B-27 supplement w/o retinoic acid	Invitrogen	12587-010
β-Mercaptoethanol	Life Technologies	21985-023
BMP4	Peprotech	120-05
Bovine serum albumin (BSA)	Sigma	A1470
Characterized fetal bovine serum	Hyclone	SH30071.03HI
CHiR 99021	Tocris	4423
CSF-1 recombinant human protein	Thermo Fisher Scientific	PHC9504
Cytosine β-D-arabinofuranoside (Ara-c)	Sigma-Aldrich	C1768
D-(+)-Glucose	Sigma-Aldrich	G8270
DAPI (4′,6-diamidino-2-phenylindole, dilactate)	Thermo Fisher Scientific	Cat# D3571; RRID:AB_2307445
Dispase (1 U/mL)	STEMCELL Technologies	07923
DMEM/F12	Gibco	11320-033
Doxycycline hydrochloride	Sigma-Aldrich	D3072
FGF-2	R&D Systems	233-FB-025
Flt3L	Peprotech	300-19
Glutamax	Life Technologies	35050-061
Hams F-12 medium	Corning	10-080-CV
HyClone RPMI 1640 media	GE Healthcare Life Sciences	SH30027.01
IMDM	Thermo Fisher Scientific	12440053
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane	Sigma-Aldrich	L2020
Matrigel GFR	Corning	354230
Monothioglycerol (MTG)	Sigma-Aldrich	M6145
N2 supplement	Invitrogen	17502-048
N2 supplement B	STEMCELL Technologies	07156
Neurobasal-A	Invitrogen	A24775-01
Penicillin/streptomycin	Thermo Fisher Scientific	15140-148
Polybrene	Sigma-Aldrich	TR-1003
Primocin	Invivogen	Ant-pm-2
Puromycin dihydrochloride	Sigma-Aldrich	P9620
Recombinant human/murine/rat BDNF	Peprotech	450-02
Recombinant human EGF	R&D Systems	236-EG-200
Recombinant human IL-34	R&D Systems	5265-IL-010
Recombinant human NT-3	Peprotech	450-03
Recombinant human TGF-β1	Peprotech	100-21
RPMI 1640	Corning	10-041-CV
RPMI 1640 media	GE Healthcare Life Sciences	SH30027.01
Sodium pyruvate	Sigma-Aldrich	P5280
SCF	Peprotech	300-07
Stem MACS iPS-Brew XF media, human	Miltenyi Biotec	130-104-368
StemPro-34 SFM (1×)	Invitrogen	10639011
StemPro Accutase	Thermo Fisher Scientific	A11105-01
VEGF165	Peprotech	100-20
Y27632	Stemgent	04-2012
Other
CellBIND 24 well clear multiple well plates, flat bottom, with lid, sterile	Corning	3337
Falcon 6 well flat-bottom plates with lids	VWR	62406-161
Falcon 24 well flat-bottom plates with lids	VWR	21100-004
Falcon 100 mm TC-treated cell culture dish	Corning	353003
Nunc Lab-Tek II chamber slide system, sterile, Thermo Scientific (8 well chamber slide)	VWR	62407-296