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. 2020 Dec 11;1(3):100197. doi: 10.1016/j.xpro.2020.100197
REAGENT or RESOURCE SOURCE IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins

Dulbecco's modified Eagle’s medium (DMEM) VWR Cat#12-614F
Fetal bovine serum (FBS) Gemini Cat#100-106
Penicillin/streptomycin VWR Cat#45000-652
Collagenase from Clostridium histolyticum Type IA Sigma-Aldrich Cat#C9891
Bovine serum albumin (BSA) Gemini Cat#700-105P
Hank’s balanced salt solution (HBSS) VWR Cat#12001-520
Phosphate buffered saline (PBS) VWR Cat#12001-676
Improved minimum essential medium (IMEM) Invitrogen Cat#10373-017

Critical Commercial Assays

Human mesenchymal stem cell (hMSC) adipogenic differentiation medium Lonza Cat#PT-3004

Experimental Models: Cell Lines

Human: MCF7 ATCC HTB-22
Human: MDA-MB-231 ATCC HTB-26

Other

50 mL centrifuge tubes VWR Cat#89039-658
20 mL syringe VWR Cat#BD302830
0.20 μm pore syringe filter VWR Cat#28144-040
Petri dishes VWR Cat#25388-606
Scalpels VWR Cat#25863-222
Forceps VWR Cat#89259-944
Parafilm VWR Cat#52858-076
200 μm cell strainer Pluriselect Cat#43-50200-03
18G needles VWR Cat#89234258
70 μm cell strainer VWR Cat#102095-534
6-well plates VWR Cat#82050-842
100 mm cell culture dishes VWR Cat#82050-916
150 mm cell culture dishes VWR Cat#25383-103
Table top centrifuge for 50 mL tubes Eppendorf Cat#5811000620
Vertical tube rotator for 50 mL tubes VWR Cat#10136-084

Biological Samples

Human breast tissue n/a n/a