Chemicals, Peptides, and Recombinant Proteins |
|
Dulbecco's modified Eagle’s medium (DMEM) |
VWR |
Cat#12-614F |
Fetal bovine serum (FBS) |
Gemini |
Cat#100-106 |
Penicillin/streptomycin |
VWR |
Cat#45000-652 |
Collagenase from Clostridium histolyticum Type IA |
Sigma-Aldrich |
Cat#C9891 |
Bovine serum albumin (BSA) |
Gemini |
Cat#700-105P |
Hank’s balanced salt solution (HBSS) |
VWR |
Cat#12001-520 |
Phosphate buffered saline (PBS) |
VWR |
Cat#12001-676 |
Improved minimum essential medium (IMEM) |
Invitrogen |
Cat#10373-017 |
|
Critical Commercial Assays |
|
Human mesenchymal stem cell (hMSC) adipogenic differentiation medium |
Lonza |
Cat#PT-3004 |
|
Experimental Models: Cell Lines |
|
Human: MCF7 |
ATCC |
HTB-22 |
Human: MDA-MB-231 |
ATCC |
HTB-26 |
|
Other |
|
50 mL centrifuge tubes |
VWR |
Cat#89039-658 |
20 mL syringe |
VWR |
Cat#BD302830
|
0.20 μm pore syringe filter |
VWR |
Cat#28144-040 |
Petri dishes |
VWR |
Cat#25388-606 |
Scalpels |
VWR |
Cat#25863-222 |
Forceps |
VWR |
Cat#89259-944 |
Parafilm |
VWR |
Cat#52858-076 |
200 μm cell strainer |
Pluriselect |
Cat#43-50200-03 |
18G needles |
VWR |
Cat#89234258 |
70 μm cell strainer |
VWR |
Cat#102095-534 |
6-well plates |
VWR |
Cat#82050-842 |
100 mm cell culture dishes |
VWR |
Cat#82050-916 |
150 mm cell culture dishes |
VWR |
Cat#25383-103 |
Table top centrifuge for 50 mL tubes |
Eppendorf |
Cat#5811000620 |
Vertical tube rotator for 50 mL tubes |
VWR |
Cat#10136-084 |
|
Biological Samples |
|
Human breast tissue |
n/a |
n/a |