Reagent	Amount
Purified gDNA (from step 17)	1 μL
DreamTaq DNA polymerase buffer 10×	1.5 μL
10 mM each dNTPs mix	0.3 μL
10 μM 1514 primer	0.3 μL
10 μM 3459 primer	0.3 μL
DreamTaq DNA polymerase	0.075 μL
ddH2O	11.525 μL
Total	15 μL