| Reagent | Amount |
|---|---|
| 2 μg gDNA (from step 17) | X μL |
| CutSmart buffer 10× | 15 μL |
| 1:10 diluted SAM | 2 μL |
| MmeI (2 U/μL) | 3 μL |
| ddH2O | 130–X μL |
| Total | 150 μL |
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