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. 2020 Dec 4;1(3):100194. doi: 10.1016/j.xpro.2020.100194

Figure 4.

Figure 4

Expected Outcomes of the Chronic Cranial Window Surgery

(A) Image of the cranial window 7–60 days after the surgery. The same mouse as in Figure 2.

(B) A mouse, head-fixed on the imaging setup.

(C) Two-photon images (right) and reconstruction of dLGN thalamocortical axons (labeled by AAV injection for TurboRFP expression in dLGN) as well as retrogradely transported beads (injected into dLGN) in cortical layer 6. The reconstruction spans full cortical depth (900 μm; left; adapted from Augustinaite and Kuhn, 2020). dLGN, dorsal lateral geniculate nucleus. The orientation: a, anterior; p, posterior; l, lateral; m, medial.