Figure 2.

pDonor Plasmid Generation and General Schematic

Protocol steps schematic: MADR pDonor TagBFP2-3Flag (cyto) WPRE plasmid can be opened by EcoRI and NotI digestion and used as a backbone to clone the sequences of interest which can be obtain by PCR from a cDNA from a source of expression of the sequence of interest or from a plasmid where the sequence was already cloned. By NEB assembly the new MADR pDonor containing the sequences of interest can be generated and validated both in vitro and in vivo