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| Step | Temperature | Time |
|---|---|---|
| DNA denaturing | 98°C | 1–5 min (depending on the template size) |
| Annealing∗ | Primer dependent | 30 s |
| Elongation∗ | 72°C | 1 min/kb of sequence to amplify |
| DNA denaturing∗ | 98°C | 30 s |
| Final elongation | 72°C | 5 min |
| Cool down | 4°C | Until recover of samples |
∗35–40 cycles.
