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. 2020 Nov 26;1(3):100191. doi: 10.1016/j.xpro.2020.100191

Figure 1.

Figure 1

Whole Mouse Organ Clearing with CUBIC Reagents

(A) The colored 50% CUBIC-L after gently shaking at 37°C overnight (after step 6).

(B) The 3D staining of intestine with flat bottom containers (step 9).

(C) The images of lung and brain at each step (most left, after fixation; second left, after delipidation and decolorization with CUBIC-L; second right, after RI adjustment with CUBIC-R (N); most right, after PBS wash with transparent organs).

(D) The image of transparent lung stained with RedDot2 (after steps 12 and 13).