Figure 2.

Whole Organ Imaging with the Experimental Lung Metastasis Model

A549-mCherry-Luc2 cells were pre-treated with TGF-β1 for 2 days. These cells were injected intravenously (iv) into nude mice. Ten days after iv injection, mice were sacrificed and excised lungs were subjected to CUBIC procedures. The 3D images (A) and 2D images (B) were analyzed using Imaris software (A), (B) or ImageJ (B).