Standard amounts of reagents and times of shaking

Organ	Amounts of CUBIC-L and CUBIC-R (N) used	CUBIC-L
Lung	10–20 mL	1–3 days
Brain	20–30 mL	2–4 days
Liver	30–40 mL	5–7 days
Kidney	10–20 mL	2–4 days
Heart	10–20 mL	2–4 days