Reagent or Resource	Source	Identifier
Antibodies
Monoclonal anti-actin, α-smooth muscle-FITC antibody	Sigma-Aldrich			Cat#F3777
RedDot2	Biotium Inc			Cat#40061
Anti-E-cadherin (24E10) antibody	Cell Signaling Technology			Cat#3195
Chemicals, Peptides, and Recombinant Proteins
Hydrogen peroxide	Wako			Cat#081-04215
Methanol	Nacalai Tesque			Cat#21915-93
Universal HIER antigen retrieval reagent (10×)	Abcam			Cat#ab208572
Blocking One	Nacalai Tesque			Cat#03953-95
VECTASTAIN Elite ABC Rabbit IgG Kit	VECTOR Laboratories			Cat#PK-6101
ImmPACT DAB	VECTOR Laboratories			Cat#SK-4105
Polyethylene glycol mono-p-isooctylphenyl ether/TritonX-100	Nacalai Tesque	Cat#12967-45
N-Butyldiethanolamine	Tokyo Chemical Industry	Cat#B0725
CUBIC-L (for animals)	Tokyo Chemical Industry	Cat#T3740
2,3-Dimethyl-1-phenyl-5-pyrazolone/antipyrine	Tokyo Chemical Industry	Cat#D1876
Nicotinamide	Tokyo Chemical Industry	Cat#N0078
CUBIC-R+ (for animals)	Tokyo Chemical Industry	Cat#T3741
HIVAC-F4	Shin-Etsu Chemical
Mineral oil	Sigma-Aldrich	Cat#M8410
Mounting solution (for CUBIC-R+)	Tokyo Chemical Industry	Cat#M3294
Blocker Casein in PBS	Thermo Fisher Scientific	Cat#37528
Sodium azide	Nacalai Tesque	Cat#31208-82
Paraformaldehyde	Wako	Cat#162-16065
Osteosoft	Merk Millipore	Cat#101728
PBS (phosphate-buffered saline) (pH 7.4)	n/a	n/a
TBS (Tris-buffered saline)	n/a	n/a
PB (phosphate buffer)	n/a	n/a
Pentobarbital	Kyoritsu Seiyaku	n/a
Isoflurane	FUJIFILM Wako Pure Chemical Corporation	Cat#099-06571
Software
Imaris software	https://imaris.oxinst.com/		n/a
ImageJ FIJI	https://imagej.nih.gov/ij/ https://fiji.sc/		n/a
Other
Light sheet fluorescence microscope (LSFM)	Olympus, custom-made	n/a
Confocal microscope	Olympus	FLOVIEW FV1200
In vivo imaging system	Berthold Technologies PerkinElmer	Night OWL IVIS (Spectrum, Lumina)
Three-way stopcock	TERUMO	Cat#TS-TR2K
30 mL syringe	Nipro	Cat#08-853
Shaker	n/a	n/a
RI measurement equipment	ATAGO	Cat#DR-A1
Paraffin-embedded block manufacturing equipment	n/a	n/a